Table 3 Parameters used for the precomputation of siRNAs.
Design parameters | Value |
|---|---|
Percent GC Content range | Moderate range, 30% to 50% |
Multiple nucleotide run (Runs of 4 or more nucleotides) | Avoided |
Open reading frame | An open reading frame region between 50 nucleotides from the 5' end and 50 nucleotides from the 3' end, downstream and upstream of the mRNA was considered for the design. |
Score cutoff | Score > 50. siRNAs with scores > 50 were retained |
Blast Hit cutoff | Number of blast hits < = 2. siRNAs with blast hits < = 2 and percent homology > 80% were retained. |