sIR: siRNA Information Resource, a web-based tool for siRNA sequence design and analysis and an open access siRNA database

Table 1 Weights of the scoring system

No	Criteria for the scoring system	Weight(W)
A1	A/U at position 1 of sense strand	-1.4
A2	G/C at position 1 of sense strand	1.11
A3	A at position 6 of sense strand	0.70
A4	U at position 10 of sense strand	0.25
A5	G at position 13 of sense strand	-1.66
A6	U/T at position 13 of sense strand	0.31
A7	A/u at position 16 of sense strand	0.74
A8	A/U at position 17 of sense strand	1.20
A9	A/u at position 18 of sense strand	1.44
A10	A/U at position 19 of sense strand	0.87
A11	G/C at position 19 of sense strand	-1.02
A12	GC content	0.42

This table shows the different weights converged upon for the various parameters using a MATLAB algorithm. It can be observed that the criterion A5 with a base 'G' at position 13 of sense strand has the highest absolute weight whereas the criterion A4 with a base 'U' at position 10 of sense strand has the lowest absolute weight. The parameters with positive weights enhance the siRNA efficacy whereas the ones with negative weights suppress the efficacy of siRNA.

ISSN: 1471-2105