Oligo length | Procedure | Sensitivity factor | Success rate (%)a | Cross homology b | Execution time (hours) |
---|---|---|---|---|---|
70-mer | IAB c | 0.2 | 94 | 0.56 | 0.50 |
 |  | 0.3 | 95 | 0.56 | 0.71 |
 |  | 0.4 | 95 | 0.56 | 0.90 |
 | BLAST with U d d | 95 | 0.56 | 2.13 | |
 | Pure BLAST | 95 | 0.61 | 3.69 | |
50-mer | IAB | 0.2 | 91 | 0.64 | 0.20 |
 |  | 0.3 | 93 | 0.64 | 0.31 |
 |  | 0.4 | 93 | 0.64 | 0.32 |
 | BLAST with U d | 93 | 0.64 | 0.70 | |
 | Pure BLAST | 93 | 0.69 | 2.19 | |
25-mer W = 11e | IAB | 0.2 | 94 | 0.79 | 0.19 |
 |  | 0.3 | 94 | 0.79 | 0.26 |
 |  | 0.4 | 94 | 0.79 | 0.33 |
 | BLAST with U d | 94 | 0.79 | 0.78 | |
 | Pure BLAST | 94 | 0.80 | 1.62 | |
25-mer W = 8 | IAB | 0.2 | 93 | 0.80 | 0.36 |
 |  | 0.3 | 93 | 0.80 | 0.50 |
 |  | 0.4 | 93 | 0.80 | 0.64 |
 | BLAST with U d | 93 | 0.80 | 1.52 | |
 | Pure BLAST | 93 | 0.81 | 3.51 | |
25-mer W = 5 | IAB | 0.2 | 92 | 0.81 | 1.00 |
 |  | 0.3 | 92 | 0.81 | 1.39 |
 |  | 0.4 | 92 | 0.81 | 1.79 |
 | BLAST with U d | 92 | 0.81 | 4.25 | |
 | Pure BLAST | 92 | 0.81 | 9.69 |