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Figure 4 | BMC Bioinformatics

Figure 4

From: The Deuterator: software for the determination of backbone amide deuterium levels from H/D exchange MS data

Figure 4

Main Spectral Viewer. Top. Co-added low-resolution spectral data within the selected retention time range is presented in the viewer. The theoretical isotopic envelope (blue) is overlaid on the observed data (red), showing the best-fit envelope used for calculation of deuterium content. Bottom. Co-added high-resolution spectral data within the selected retention time range is presented in the viewer. Correct peak data is extracted from calculated sub-ranges only, displayed as yellow bars. Correct peaks are extracted from the spectral data, enabling more accurate centroid measurements in areas with poor signal to noise ratios or overlapping peptides. 'A' Peaks belong to the peptide of interest and all reside within the sub ranges. 'B' peaks belong to another peptide (+1), which will be disregarded from centroid calculations.

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