Table 3 Comparison of annotations of PPIs common to literature-derived curated PPI databases
From: An evaluation of human protein-protein interaction data in the public domain
Interacting Proteins | HPRD | DIP | BIND | MIPS | MINT | IntAct | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Detection method | PubMed ID | Detection method | PubMed ID | Detection method | PubMed ID | Detection method | PubMed ID | Detection method | PubMed ID | Detection method | PubMed ID | |||
1 | NFKB1 | NFKB3 | in vivo | 9101089 | Tandem Affinity Purification (TAP) | 14743216 | Gel retardation assays, three dimensional structure | 15735750, 9738011, 9865693 | - | - | anti tag coimmuno- precipitation | 14743216 | Comigration in gel, anti bait coip, tap | 8246997, 8246997, 14743216 |
2 | TNFRSF1A | TRADD | in vivo, in vitro, Yeast 2-hybrid | 7758105, 8565075, 8612133 | Experimental | 9129204 | Immuno- precipitation | 11684708, 15247912, 9916731 | coip: coimmuno precipitation | 9916731 | Coimmuno- precipitation, pull down, two hybrid | 8565075, 8621670 | anti bait coip, pull down, two hybrid | 7758105 |
3 | FADD | FAS | in vivo, in vitro, Yeast 2-hybrid | 8967952, 7538907, 7536190 | Two hybrid test | 7538907 | Immuno- precipitation | 15665818, 15383280 | coip: coimmuno precipitation | 10196099 | Coimmuno-precipitation, two hybrid | 7536190, 7538907 | anti tag coip, coip, pull down, two hybrid | 7538907, 7536190, 7538907, 7538907 |
4 | PEX19 | PEX3 | in vivo, in vitro, Yeast 2-hybrid | 10704444, 12096124 | - | - | two-hybrid-test | 10430017, 12096124 | coip: coimmuno precipitation, two hybrid | 10430017 | two hybrid, ubiquitin reconstruction | 12096124, 16189514 | far western blotting, two hybrid pooling | 10704444, 16189514 |
5 | CDK2 | CDKN1A | in vitro | 12839982 | Two hybrid test | 8242751 | other | 15232106 | coip: coimmuno precipitation | 8641969 | protein array, pull down | 15232106, 9284049 | protein array, pull down | 15232106, 8756624 |
6 | PEX12 | PEX5 | in vivo, in vitro, Yeast 2-hybrid | 10562279, 10837480, 12096124 | - | - | two-hybrid-test | 12096124 | coip: coimmuno precipitation, two hybrid | 10646847 | two hybrid | 12096124 | anti tag coip, filter binding, two hybrid | 10562279, 10562279, 12620231 |