Figure 10
From: CLPM: A Cross-Linked Peptide Mapping Algorithm for Mass Spectrometric Analysis
SDS-PAGE analysis of NS3h-deoxynucleotide photocrosslink. The crosslink reaction was initiated by exposing the sample to 305 ± 16 nm UV light for three hours. The concentration of DNA was 1 uM and the NS3h concentration was 3 uM. After crosslinking, the sample was separated by SDS-PAGE (12% acrylamide gel). Fluorescent image was taken before (A) and after (B) Commassie staining. Horizontal arrows identify the crosslinked proteins that are 10 kDa heavier than uncrosslinked proteins. Lanes a and i are molecular markers, b is NS3h without adding deoxynucleotide, and c is NS3h and deoxynucleotide without UV light excitation. Lanes d, e, f, and g are NS3h plus deoxynucleotide with three hours activation by 305 nM UV light. Lane h was unused and served as a negative control.