Figure 3

Comparison of AutoFACT annotations across four phylogenetically diverse organisms previously annotated by well-established automatic pipelines. Two hundred previously annotated cDNAs from Homo sapiens [Ensembl Annotation Pipeline], Saccharomyces cerevisiae [MIPS/PEDANT], Plasmodium falciparum [TIGR] and Rickettsia prowazekii [GeneQuiz] were re-annotated with AutoFACT using a bit score cutoff of 40 and a database order of importance as follows: UniRef90, KEGG, COG, NCBI's nr, Pfam and SMART. The top 10 BLAST hits to each database were filtered for functionally uninformative terms. BLAST hits to the species itself were considered uninformative. The portion of the bar representing different results from AutoFACT (dark purple) should not be construed as false positives. For example in the case of GeneQuiz (4.5% differences), it is the AutoFACT annotation that is the better of the two in almost all instances (see Results section). Numbers printed directly on columns represent the number of cDNA sequences (out of 200) in each category.