Figure 2

Sample HTML output for AutoFACT annotation of Acanthamoeba castellanii EST cluster ACL00000152. Automatic annotation results are displayed at the top of the page and all data used to infer the annotation are represented in the bottom part of the table. Percent sequence identity is the extent to which two (nucleotide or amino acid) sequences, in a High Scoring Segment Pair (HSP), are invariant. In the case of the est_others data, the reported % sequence identity refers to a "translated nucleotide – translated nucleotide" comparison. The "Informative Hit" value specifies whether the first, second, etc., BLAST hit in the corresponding database was informative. The "Color Key for Alignment Scores" displayed at the top of the diagram is from NCBI's BLAST Results page. The scores for the annotation and for the source of the annotation, 627 in this example, are highlighted according to the color key. The page also contains links to relevant database entries.