Table 3 Promiscuous validation test. All 24 sequences of promiscuous coupled GPCR subtypes (as summarized in [37] and other sources [38]) were parsed in an hmmpfam query against our refined library of profile Hidden Markov models (pHMMs). The query sequences were not included in the training set. Promiscuous coupling was correctly predicted for 6 queries.
GPCR Subtype | Uniprot AC | observed | predicted |
|---|---|---|---|
Alpha-2A adrenergic receptor | P08913 | Gq/11 Gi/o Gs | Gq/11 Gi/o Gs |
AT1 angiotensin receptor | P30556 | Gq/11 Gi/o | Gq/11 Gi/o |
Beta-3 adrenenergic receptor | P25962 | Gi/o Gs | Gq/11 Gio Gs |
C3A anaphylatoxin chemotactic receptor | Q16581 | Gq/11 Gi/o | Gi/o |
Calcitonin receptor | P30988 | Gq/11 Gs | Gs |
Cholecystokinin type 1 receptor | P32238 | Gq/11 Gs | Gq/11 Gi/o |
Sphingosine 1-phosphate receptor 3 (EDG3) | Q99500 | Gq/11 Gi/o | Gi/o |
Lysophosphatidic acid receptor 2 (EDG4) | Q9HBW0 | Gq/11 Gi/o | Gi/o |
Endothelin B receptor | O60883 | Gq/11 Gi/o | Gi/o |
Endothelin A receptor | P25101 | Gq/11 Gs | Gq/11 |
Follicle-stimulating hormone receptor | Q95179 | Gi/o Gs | Gs |
Galanin receptor type 2 | O43603 | Gq/11 Gi/o | Gq/11 Gi/o |
Leukotriene B4 receptor | Q15722 | Gq/11 Gi/o | Gq/11 Gi/o |
Lutropin-choriogonadotropic hormone receptor | P22888 | Gq/11 Gi/o Gs | Gs |
Neuromedin K receptor (NK-3) | P29371 | Gq/11 Gs | Gq/11 Gi/o |
Neuropeptide Y receptor type 1 | O02813 | Gq/11 Gi/o | Gi/o |
Oxytocin receptor | P30559 | Gq/11 Gi/o | Gq/11 Gi/o |
P2Y purinoceptor 11 | Q96G91 | Gq/11 Gs | Gi/o |
Platelet-activating factor receptor | Q62035 | Gq/11 Gi/o | Gq/11 Gi/o |
Prostaglandin E2 receptor EP3 | P43115 | Gq/11 Gi/o Gs | Gi/o |
Substance-K receptor (NK-2) | P21452 | Gq/11 Gs | Gq/11 Gi/o |
Substance-P receptor (NK-1) | P25103 | Gq/11 Gs | Gq/11 Gi/o |
Proteinase activated receptor 1 (thrombin receptor) | P25116 | Gq/11 Gi/o | Gi/o |
Thyrotropin receptor | P47750 | Gq/11 Gi/o Gs | Gi/o Gs |