Figure 2

Phylogenetic tree and domain architecture of the protein families bearing the PAM domain. The tree was built using the corresponding PAM domain region of the CSN2, Rpn3, Thp1 proteins in representative species. Where uncharacterized, the proteins are indicated with their database accession number. The nodes with a bootstrap support exceeding 90% are indicated with coloured lines, those with a bootstrap value below 90% are reported in black. The domain architecture was derived from the SMART database [28]. A divergent version of the PCI/PINT domain is indicated in yellow (Table 1, see Additional data). It should be noted that the N. crassa EAA35198 protein is a hypothetical protein derived from gene prediction. As it is the only protein with a PAM domain showing the thiolase domains, it could be that the gene prediction is wrong and the actual protein is bearing only the PAM domain. Abbreviations: PINT: P roteasome, I nt-6, N ip-1 and T RIP-15; PAM, P CI/PINT a ssociated m odule; Thiolase: Thiolase, N-terminal domain; Thiolase_C: Thiolase, C-terminal domain.