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Figure 4 | BMC Bioinformatics

Figure 4

From: Simulation of DNA array hybridization experiments and evaluation of critical parameters during subsequent image and data analysis

Figure 4

Pin shifting. Every block was shifted randomly relative to its ideal position by a Gaussian distributed distance with a given standard deviation σ. (A) simulated image, σ = 0.114 mm, (B) Pearson correlation of simulated and calculated intensities dependent on the standard deviation of the block centers from their ideal positions (for AIDA and Visual Grid each data point is determined by a single analysis of a simulated image and for FA three different images has been analyzed for each σ, the asterisk is the mean and the error bar shows the minimum and maximum value of the three repetitions), (C) mean distance between the calculated and simulated spot centers dependent on the standard deviation of the block centers from their ideal positions (each data point is determined by a single analysis of a simulated image). The vertical lines in (B) and (C) correspond to the simulated image in (A).

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