Table 4 Top 5 predictions of each type for the first group of 60 "ambiguous" annotated proteins.

Correct

YBL034C

ambiguous spindle pole

spindle pole

Nucleus. Spindle.

cytoskeleton. kinetochore.

spindle pole body (IDA)

YDR181C

ambiguous; cytoplasm;

cytoplasm;

Nucleus

nuclear chromatin (IDA) nuclear chromosome, telomeric region (IC)

YGR020C

ambiguous; vacuolar membrane

vacuolar membrane

fungal-type vacuole membrane (TAS) vacuolar proton-transporting V-type ATPase, V1 domain (TAS)

YHR119W

ambiguous; nucleus

nucleus

Nucleus (Probable). Chromosome (Probable).

Set1C/COMPASS complex (IPI)

YHR183W

ambiguous; cytoplasm

cytoplasm

Cytoplasm

cytoplasm (IDA)

mitochondrion (IDA)

Partial Correct

YAL029C

ambiguous;

cell periphery; bud neck; cytoplasm;bud

Bud

Bud

cellular bud (IDA)

cellular bud tip (IDA)

filamentous actin (IDA)

mitochondrion (IDA)

YBR102C

ambiguous;

cell periphery;

bud neck;bud

cytoplasm;

bud

secretory vesicle.

Bud. Bud neck.

cellular bud neck (IDA)

cellular bud tip (IDA)

YBR130C

ambiguous;

cell periphery; cytoplasm;bud

bud

actin cap (TAS)

cellular bud tip (IDA)

cytoplasm (IDA)

YBR260C

ambiguous;

cytoplasm;

bud;bud neck;

cytoplasm

Cytoplasm.

actin cortical patch (IDA)

cellular bud (IDA)

mating projection tip (IDA)

YFR016C

ambiguous;

cytoplasm;bud

cytoplasm

cellular bud (IDA)

cytoplasm (IDA)

Mismatch

YAR019C

ambiguous; spindle pole

cytoplasm

cellular bud neck (TAS)

spindle pole body (IDA)

YBL105C

ambiguous;

cytoplasm;

bud neck;bud

actin

cytoplasm (IDA)

cytoskeleton (IDA)

nucleus (IDA)

YDL146W

ambiguous;

cell periphery;

cytoplasm;

bud neck;bud

actin

Bud.

Cytoplasm

Bud neck

colocalizes with actin cortical patch (IDA)

cellular bud (IDA)

cytoplasm (IDA)

cellular bud neck (IDA)

YDR309C

ambiguous;

cytoplasm;

bud

actin

Bud neck (By similarity).

Bud tip (By similarity).

cell cortex (By similarity)

cytoskeleton (By similarity).

actin cap (TAS)

cellular bud tip (IDA)

incipient cellular bud site (IDA)

mating projection tip (IDA)

plasma membrane (IGI)

YHR158C

ambiguous;

cell periphery; bud neck; bud

cytoplasm;

nucleus

cellular bud neck (IDA)

cellular bud tip (IDA)

mating projection tip (IDA)

cytoplasm (IDA)

YCL024W

ambiguous;

cell periphery; bud neck;bud

Bud neck

cellular bud neck (IDA)

cellular bud neck septin collar (IDA)

incipient cellular bud site (IDA)

YDL089W

ambiguous;

nuclear periphery

Membrane

nuclear periphery (IDA)

Unknown

YDR069C

ambiguous;

endosome

Cytoplasm.

Late endosome membrane;

endosome (IDA)

membrane fraction (IDA)

proteasome complex (IPI)

mitochondrion (IDA)

YDR507C

ambiguous;bud

bud neck;cytoplasm

Cytoplasm.

Bud neck.

cellular bud neck (IDA)

YHL019C

ambiguous;

late Golgi

coated pit.

AP-1 adaptor complex (IPI)