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Table 8 Combination of TAGs with Primers — TAG-primer pairing

From: EGNAS: an exhaustive DNA sequence design algorithm

 

Flanking

TAG-primer pairs

 

sequences

Fan et al.

EGNAS

Average ΔG

-0.7 ± 1.2

-2.8 ± 1.4

-1.2 ± 1.1

Minimum ΔG

-4.3

-9.0

-4.3

  1. Comparison of SBE-TAGs used by Fan et al.[8] and those generated by EGNAS. Molar free enthalpies (ΔG, kcal/mol) of the most stable hairpins. Averages ± standard deviations from 148 sequences.
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BMC Bioinformatics

ISSN: 1471-2105

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