Skip to main content

Table 3 ROCS results for the number of Indicator Prey Protein s, Reproducible Experimental Replicates , and joint inclusion probability p ^ J at various experimental scales K in each AP-MS control and bait experiments: STK24, PPM1B, NME2, CTNNBIP1, VHL, and CONTROL

From: ROCS: a Reproducibility Index and Confidence Score for Interaction Proteomics Studies

Bait Experiment with experimental

scale K( K C or K B )

Q ^ B or Q ^ C

# Total Indicator Prey Proteins

p ˜ min B or p ˜ min C

Bait Marginal Inclusion Probability Threshold

Q ^ B ( p ˜ min B ) or Q ^ C ( p ˜ min C )

# Selected Indicator Prey Proteins

(% of Q ^ B or Q ^ C )

L ^ B ( p ˜ min B ) or L ^ C ( p ˜ min C )

# Reproducible Experimental Replicates

(% of K C or K B )

p ^ J B ( p ˜ min B ) or p ^ J C ( p ˜ min C )

Joint Inclusion Probability

5 (STK24)

145

0.80

24 (16.6%)

3 ( 60.0%)

0.007

6 (PPM1B)

71

0.80

7 ( 9.9%)

3 ( 50.0%)

0.480

8 (NME2)

145

0.80

4 ( 2.8%)

8 (100.0%)

1.000

9 (CTNNBIP1)

107

0.75

11 (10.3%)

5 ( 55.5%)

0.540

33 (VHL)

597

0.75

15 ( 2.5%)

11 ( 33.3%)

0.070

200 (CONTROL)

542

0.75

4 ( 0.7%)

124 ( 62.0%)

0.550

  1. Results are reported for the marginal inclusion probability thresholds p ˜ min C and p ˜ min B as determined in each AP-MS bait experiment.