Nucleotide | A | C | T | G | Total f | Per cycle p |
---|
A | 0.9995 | 7.2e-6 | 7.7e-6 | 5.1e-4 | 5.2e-4 | 3.5e-5 |
C | 1.1e-05 | 0.9996 | 4.1e-4 | 2.1e-6 | 4.2e-4 | 2.8e-5 |
T | 9.0e-6 | 5.7e-4 | 0.9994 | 1.4e-5 | 5.9e-4 | 4.0e-5 |
G | 3.5e-4 | 3.2e-6 | 2.1e-5 | 0.9996 | 3.7e-4 | 2.5e-5 |
- The rows give the true nucleotides and the columns the observed as a result of errors. Estimates of the individual probabilities are given as frequencies. The total error probability f for each nucleotide is also quoted in the penultimate column, followed by the per cycle error rate p calculated as p = 2f /n, where n = 30 is the cycle number [8, 17].