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Figure 8 | BMC Bioinformatics

Figure 8

From: Removing Noise From Pyrosequenced Amplicons

Figure 8

Training logistic regression on denoised V2 'Even' data. Good sequences are shown as black dots, chimeras red and reference sequences magenta. We used the three denoised V2 'Even' data sets, classified either good or chimeric by comparison with the references, and the reference sequences, all good, to train a one dimensional logistic regression on the 'chimera index' I using the R software package. An intercept, α = - 2.83542, and coefficient β = 0.55889 were obtained (highly significantly different from zero). The corresponding P25, P50 and P75 decision lines are shown (blue lines). The fit reduced the null deviance from 1371.81 on 5416 degrees of freedom to a residual deviance of 416.36 on 5415 degrees of freedom (AIC 420.36).

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