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Figure 3 | BMC Bioinformatics

Figure 3

From: Addressing the Challenge of Defining Valid Proteomic Biomarkers and Classifiers

Figure 3

Number of significant markers depends on sample size. From the 2 × 67 training data, data sets of sizes Ndiff ranging from 7 to 67 were built via resampling. At each sample size the number of significant biomarkers (defined as having a p-value after BH adjustments < 0.05) is shown on the vertical axis. The procedure was repeated 10 times to generate the Box-Whisker plots. In all 10 experiments, no biomarkers could be declared significantly differentially expressed below a sample size of 13. On the top left, populations of sizes up to 480 were generated using resampling with replacement, based on the 2 × 67 samples. The figure shows that with sample sizes around 2 × 400 a plateau is reached.

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