Table 3 Parameter values used in the first application example
From: TagCleaner: Identification and removal of tag sequences from genomic and metagenomic datasets
Parameter | Value |
|---|---|
Maximum number of mismatches at 5'-end | 3 |
Maximum number of mismatches at 3'-end | 3 |
Sequence range from the ends | 46 bp |
Continuous trimming | Yes |
Remove sequences not matching tag sequence | No ("don't remove") |
Minimum read length | 50 bp |
Maximum read length | Default (maximum length) |
Threshold for occurrence of N | 5% |
Dereplicate data (remove exact sequence copies) | Yes |
Fragment-to-fragment splitting | Yes |
Maximum number of mismatches for splitting | 3 |