Figure 2

The relationship between the fractional part of bead position and observed intensity. (A) To investigate the association between bead-intensity and the fractional part of a bead's location we use the bead coordinates and intensities for array section 4343238066_A_1. A pixel is divided into 100 × 100 bins and the average log-intensity of beads with fractional coordinates falling into those bins has been plotted. (B) As for part A of the figure, but the fractional part of the coordinate was not used in the intensity calculation, with the four pixels instead being given equal weight. (C) As for part A of the figure, but within bead-type residual log-intensities are plotted. (D) As for part B of the figure, but within bead-type residual log-intensities are plotted.