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Table 1 Multi-way ANOVA table assessing components used in OTU methodologies.

From: Alignment and clustering of phylogenetic markers - implications for microbial diversity studies

Parameter

Sum of Squares

Degrees of freedom

Mean Sq.

F

Prob >F

Distance threshold

0.4411

11

0.0401

23.0160

< 0.0001

MSA

0.0480

2

0.0240

13.7843

< 0.0001

Clustering

0.0099

2

0.0050

2.8503

0.0604

Distance correction

< 0.0001

2

< 0.0001

0.0020

0.9980

Error

0.3171

182

0.0017

  

Total

0.7910

199

0.0708

  
  1. The factor with the largest effect on the quality of the OTUs was the distance threshold, followed by the MSA, and then the clustering algorithm. The distance correction explained < 0.01% of the variance and no statistically significant difference could be detected between the corrections (P = 0.998).