Figure 3
From: Alignment and clustering of phylogenetic markers - implications for microbial diversity studies
Variability in nonparametric estimators and diversity indices using a clustering distances 0.01-0.05. Plots of (a) Ace and Chao1, and (b) Shannon measures reveal significant sensitivity to OTU sets. Each plotted methodology used either the MUSCLE, ClustalW, or NAST MSA; they also used either furthest, nearest, or average neighbor clustering, and one of the following evolutionary distance corrections: JC, K2P, or F84.