Figure 1

FIRE plots. Plots represent the pairwise alignment of ω MLEs at codon sites with FIRE, recorded as a percent similarity between the two values. Corresponding FIRE scores and alignments are in TABLE 1 and additional file 3, respectively. A sliding window of 16 codons was used and the percent similarity is the average over the window. (A) conserved orthologous metazoan and protozoan MYB1 DBDs; (B) conserved paralogous metazoan MYB1 and MYB2 DBDs; (C) conserved metazoan and protozoan GK; (D) κ and λ light chain antibodies; (E) metazoan MYB1 and p53 DBDs; and (F) p53 DBD and κ light chain antibody. The sequence sets used in plots E and F have no functional similarity and represent negative controls. The 60% similarity cut-off value is indicated by a solid line. DBD = DNA-binding domain; GK = glycerol kinase.