Skip to main content

Table 3 Characterization of common de novo sequencing errors.

From: A high-throughput de novo sequencing approach for shotgun proteomics using high-resolution tandem mass spectrometry

De novo sequencing error types

Top sequence tags

Consensus sequence tags

 

Counts

Percentage

Counts

Percentage

Substitution of a Q by GA

243

9.8%

174

14.6%

Substitution of an N by GG

51

2.1%

42

3.5%

Inversion of two adjacent residues

222

9.0%

103

8.7%

Subsitution of an end residue

1002

40.5%

421

35.4%

Subsitution of an internal residue

98

4.0%

59

5.0%

Other

861

25.8%

391

32.9%

Total

2477

100.0%

1190

100.0%